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With virus expressing E3L deleted of the N terminus, vaccination with as little as 100 plaque forming units (pfu) intranasally was suf cient to protect against a wild-type vaccinia virus challenge of 106 pfu For virus deleted of E3L completely or deleted of the dsRNA binding motif, 1000 pfu was able to provide an equivalent level of protection against wild-type virus challenge Thus, these mutants can produce a mucosal or dermal protective response even though they are at least 5 log10 units less virulent than wild-type virus For virus deleted of E3L, the 1000 pfu inoculum provides effective protection yet the virus does not replicate ef ciently in the nasal tissue reaching maximal titers around 1000 pfu/g tissue.

Usually, as will be shown in the next section, to estimate a position, a node uses at least three distance estimations, each of them with an associated error On the other hand, if only the accuracy of such methods were important, we could just use a TDoA that has lower errors But factors such as the size and cost (in terms of hardware, processor, and energy) of the nodes must also be taken into consideration Thus, the chosen method used to estimate distances will depend on the application requirements and also on the available resources Table 112 compares each one of the methods described in this section..

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In Symbian OS v8.0, we added support for transparent windows. This feature requires that WSERV be able to draw the content of windows upon demand. When the client draws a window, WSERV stores the redraw commands. It does this for all windows, not just windows that might be involved with transparency. The advantage of this to the client is that, so long as it doesn t change the content of the window, it doesn t have to redraw that window each time part of it becomes invalid WSERV will just reprocess the commands that it has stored. However, if the content of the window does change, then the client must tell WSERV, so that it can discard the commands

With the loss of the N terminus of E3L, the 100 pfu inoculation required for effective protection leads to ef cient virus replication in the nasal turbinates with virus titers reaching 1 106 pfu/g of tissue This increase in virus titer is required for the protective response, since when mice are inoculated with 10 pfu of the E3L N-terminally deleted virus protection against a wild-type vaccinia virus challenge is not achieved even though virus titers reach levels of 1 105 pfu/g These results suggest that vaccinia virus containing a full deletion of E3L may be the most effective vaccine since protection does not appear to require an increase in virus titers 156 CONCLUSION.

11.3 POSITION COMPUTATION When a node has enough information of distances and/or angles and positions, it can compute its own position using one of the methods that will be studied in this section. Several methods can be used to compute the position of a node. Such methods includes trilateration, multilateration, triangulation, probabilistic approaches, bounding box, and the central position. The choice of which method to use also impacts the nal performance of the localization system. Such a choice depends on the available

it has stored. The client can do this by calling Invalidate() on the window object for the area that has changed. It s worth noting that before this feature came along, a client could rely on WSERV issuing a redraw when a window was made visible, and use this as a signal to update its content.

TABLE 11.2. Comparison of the Methods Used to Estimate Distances/Angles Between Two Nodesa Method RSSI Precision Meters (2 4 m) Maximum Distance Communication range Communication range few meters (2 10 m) Communication range Communication range Extra Hardware None Challenges Variation of the RSSI, interferences Nodes synchronization Maximum distance of work Work on small sensor nodes

Of the weapons of mass destruction, biological weapons are the most feared,97 in part because many biological weapons are infectious and can

Centimeters (2 3 cm) TDoA Centimeters (2 3 cm) AoA A few degrees (5 ) Communication Half the Range communication range

Caches are the main source of unexpected interactions that affect program execution speed. For example, a benchmark that repeatedly performs a simple operation may show a two microsecond context switch, but this is only possible because the cache is lled early in the test and remains lled throughout. On a real system where the threads being switched do actual work, the contents of the cache are displaced, which makes context switching much slower. Also, because caches contain scattered pieces of information from throughout main memory and have to be able to nd that information very quickly, they use something called tag RAM . Tag RAM is even more expensive than the rest of the cache, so to reduce the amount needed, spatial locality is exploited by organizing the cache into lines , typically of eight 32-bit words each. Each line has a single tag, and will be lled in from main memory in a single operation: an eight-word burst. It is much faster to do this than to fetch eight single

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