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The amphibian AMPs are almost universally synthesized and are stored in the neuroendocrine granular glands in the skin, from where they are released in a holocrine manner following stress, adrenergic stimulation, or injury Interestingly, some of them are also produced in the gastric mucosa and accumulate in the mucus coating the stomach surface (Bulet et al 2004) The activity spectrum of the amphibian AMPs is broad They can kill a large variety of aerobic and anaerobic bacteria, clinical isolates of multiresistant human pathogens, yeast, and lamentous fungi, and they are also ef cient against some viruses, tumor cells, and protozoa However, some of them have a rather limited activity The ef cacy of amphibian AMPs is markedly increased by the considerable synergy existing in their action Considering their toxicity against erythrocytes, most of them are highly hemolytic However, this activity is rather low or even nonexistent for some.



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Sugai M., T. Fujiwara, T. Akiyama, M. Ohara, H. Komatsuzawa, S. Inoue, and H. Suginaka (1997) Journal of Bacteriology 179, 1193 1202. Takac M. and U. Blasi (2005) Antimicrobial Agents and Chemotherapy 49, 2934 2940. Thumm G. and F. G tz (1997) Molecular Microbiology 23, 1251 1265. von Eiff C., J. F. Kokai-Kun, K. Becker, and G. Peters (2003) Antimicrobial Agents and Chemotherapy 47, 3613 3615. Wall R. J., A. M. Powell, M. J. Paape, D. E. Kerr, D. D. Bannerman, V. G. Pursel, K. D. Wells, N. Talbot, and H. W. Hawk (2005) Nature Biotechnology 23, 445 451. Walsh S., J. Kokai-Kun, A. Shah, and J. Mond (2004) Pharmaceutical Research 21, 1770 1775. Walsh S., A. Shah, and J. Mond (2003) Antimicrobial Agents and Chemotherapy 47, 554 558. Wertheim H. F., D. C. Melles, M. C. Vos, W. van Leeuwen, A. van Belkum, H. A. Verbrugh, and J. L. Nouwen (2005) The Lancet Infectious Diseases 5, 751 762. Wu J. A., C. Kusuma, J. J. Mond, and J. F. Kokai-Kun (2003) Antimicrobial Agents and Chemotherapy 47, 3407 3414. Yang X.-Y., C.-R. Li, R.-H. Lou, Y-M. Wang, W.-X. Zhang, H.-Z. Chen, Q.-S. Huang, Y.-X. Han, J.-D. Jiang, and X.-F. You (2007) Journal of Medical Microbiology 56, 71 76. Yano M. Y., Y. Doki, M. Inoue, T. Tsujinaka, H. Shiozaki, and M. Moden (2000) Surgery Today 30, 16 21. Zygmunt W. A., H. P. Browder, and P. A. Tavormina (1968) Applied Microbiology 16, 1168 1173.

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lO make paper in a paper mill. The amount of lig nin present in pulp is call ed the kappa number. A very sophisticated instrument is used to measure kappa, and the output of thi s instrument ran ges from I to 5 vo ll s, where 1 volt represe nt s a kappa number of 12 and 5 volts represents a kappa number o f 20. The pu lp mill operator has asked to have a kappa meter installed on hi s co nsole. Design a circ uit that will e mploy as input the 1- to 5-volt signa l and output the kappa number. An electronics engineer in the plant has sugges ted the circu it show n in Fi g.

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